Journal Of Investigative Dermatology

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671 Study of the molecular and functional effects of wound dressings on human dermal fibroblasts

Sun, 2019-09-01 00:00
The process of cutaneous wound healing/repair is characterized by three major phases: coagulation (A) and inflammation (B) during the first hours, followed by a regeneration and remodeling/maturation phase (C) mainly conducted by dermal fibroblasts. To promote tissue repair, there is a multitude of dressings targeting the different phases of healing including calcium alginates and high absorbency fibers. The aim of the present study was to evaluate the effects of these two wound dressings subtypes on human dermal fibroblasts.

670 Targeting inflammation, dermal matrix organization and repair process for efficient prevention and correction of striae gravidarum

Sun, 2019-09-01 00:00
Stretch marks, or striae gravidarum (SG), occur frequently during pregnancy, affecting 50-90% of women. SG are characterized by inflammatory environment, alteration of dermal matrix, particularly with disorganization of elastic fibers, and at the cell level, modification of fibroblast contractile properties. Although not physically dangerous, SG represent a major aesthetic concern for pregnant women. Therefore, there’s a need for efficient topical solutions to prevent development of SG or reduce their severity.

669 The spent culture fluid of Bifidobacterium longum, Lactobacillus rhamnosus GG and Escherichia coli Nissle 1917 inhibits the growth and biofilm formation of Pseudomonas aeruginosa

Sun, 2019-09-01 00:00
Pseudomonas aeruginosa is one of the most problematic wound associated pathogens. Infection with P. aeruginosa is associated with significant morbidity and mortality. The virulence of this bacterium together with its ability to form biofilms complicates its management. Probiotics have been reported to be effective in inhibiting pathogens of both intestinal and extra-intestinal origin. The topical use of probiotics may offer new alternatives that are efficient and safe to use. The aim of the study was to assess the potential of probiotic organisms to inhibit both the planktonic and biofilm forms of P.

668 Wound Healing capabilities of keratinocytes derived iPS cells

Sun, 2019-09-01 00:00
The skin protects the body against environmental stresses such as solar ultraviolet irradiation, environmental pollution, microbial agents, and helps to prevent fluid loss. The skin protective function can be altered following mechanical injuries or disease. Wound healing is a conserved evolutionary process including several stages, i.e., inflammation, cellular proliferation and extracellular matrix remodeling. Induced pluripotent stem cells (iPS) are reprogrammed somatic cells that have regained pluripotency capability.

667 A Novel Microcurrent Device to Improve Skin Structure and Appearance

Sun, 2019-09-01 00:00
Microcurrent technology, 25 – 500 uA direct current constant or pulsed, has been used for more than a century to treat a broad spectrum of human ailments and today the microcurrent facial has become a staple in aesthetic salons claiming cellular rejuvenation, facial toning and reduction in the appearance of lines and wrinkles. A transcutaneous electrical nerve stimulation (TENS) attachment, powered by the counter oscillating motion of a facial treatment/cleansing device, was developed to deliver low level microcurrents to facial skin.

666 Targeting perfect skin repair markers in a model of induced cellular senescence

Sun, 2019-09-01 00:00
Skin repair is a multistep process involving both epidermal and dermal compartments. Among the sequential and overlapping events that take place during the skin repair process, the regeneration of the epidermal compartment represents a critical step for a complete and effective repair, qualified as perfect skin repair. In the present study, we evaluated an extract of Rosa centifolia, prepared by a proprietary extraction progress, rich in small RNA and compounds of interest including polyphenols, reducing sugars, free amino-acids and organic acids.

665 Study of the wound healing on 3D in vitro skin models

Sun, 2019-09-01 00:00
Skin wound healing is a complex process which consists in four distinct stages: hemostasis, inflammation, proliferation and maturation. The first stage involves platelets to seal the break in the wall of the blood vessel. The second stage is an inflammatory phase to eliminate the damaged cells, pathogens, and bacteria from the wounded area. The two final stages (proliferative and maturation phases) involve fibroblasts and keratinocytes to form new collagen network and epidermis. Overall, wound healing is a complex and tightly regulated process.

664 Biological activities of traditional medicinal herbs on skin cells

Sun, 2019-09-01 00:00
In the field of cosmetic and dermatologic research, traditional medicinal plants gain interest, including medicinal herbs native from China. The present study aims to investigate the biological activity of Cohesium (COHE), an Ophiopogon Japonicus Root extract, in human epidermal keratinocytes and dermal fibroblasts. Primary cell cultures were established from human skin. Cell cytotoxicity was assessed by MTT assay after 2 to 5 days of culture with several dilutions of COHE (0.01-10%). Cell proliferation was measured by a cell cycle S phase analysis using Propidium Iodide staining.

663 Medaka fish as a good model to study skin aging by Atomic Force Microscopy

Sun, 2019-09-01 00:00
Studying skin aging to better understand age-related physiological changes or to find novel molecules that could act on aging, could be relatively long and costly. Fish animal models could be particularly appropriated since skin structure has been shown remarkably useful to investigate pigment biology for example, in normal or tumoral cells (Schartl et al.,2016). In particular, the Japanese Medaka, a small fish model with attractive experimental characteristics, may accumulate over lifespan some biomarkers currently associated with aging features in other vertebrates.

662 Bacterial infection and wound healing in an ex vivo human skin model

Sun, 2019-09-01 00:00
Skin processes excellent regeneration properties allowing its rapid healing upon dermal injury. If wounds fail to heal in an orderly and timely manner, chronic ulcers develop. To test the potential of innovate therapies to promote wound repair, relevant wound infection models are needed. Current research is mainly conducted on animal models, which often limits direct transferability to human and poses ethic issues. These limitations can be overcome by using a proprietary ex vivo human skin model, HypoSkin®, produced with leftovers from esthetic surgery.

661 Evaluating complications in below-knee skin cancer surgery after introduction of preoperative appointments: a retrospective cohort study

Sun, 2019-09-01 00:00
In this study, we evaluated if a preoperative nurse appointment reduces complication risks in high-risk below-knee skin cancer surgery. We searched the medical records of HUS Skin and Allergy Hospital’s dermatosurgery unit for all below-knee surgeries, comparing year 2016 to year 2018, when the preoperative nurse appointments for risk patients were introduced. At the appointments, risk factors were evaluated and if needed, preoperative compression was introduced. We assessed the benefits of a preoperative appointment by comparing complication risks.

660 Dermal fibroblast subpopulations as a potential cell therapy for promoting scar-free wound healing and resolving scar formation

Sun, 2019-09-01 00:00
Dermal fibroblasts are responsible for maintaining and repairing tissue integrity through the synthesis of the extracellular matrix of connective tissue. These cells arise from a homogenous population during early embryonic development in mouse skin and differentiate into heterogenous populations with unique functions in development, skin maturation and wound healing. We recently identified different fibroblast populations in adult human skin, using a combination of comparative spatial transcriptional profiling.

659 Towards functional analysis of prolylcarboxypeptidase in human skin - a regulator of alpha-MSH activity?

Sun, 2019-09-01 00:00
Prolylcarboxypeptidase (PRCP) is s serine protease that recently emerged as a regulator of the central melanocortin system, i. e. control of food intake and energy metabolism, by degrading α-MSH (1-13) into biologically inactive α-MSH (1-12). Therefore, we wondered if PRCP may also play a role in regulation of the peripheral melanocortin system. As a first step towards answering this question we examined the expression of PRCP in various cutaneous cell types at RNA and protein level in vitro. PRCP transcripts were detected in normal human melanocytes, normal human keratinocytes and human dermal fibroblasts (HDFs) from different donors as shown by endpoint reverse transcriptase- polymerase chain reaction (RT-PCR).

658 Modulating NFkB signaling in skin wounds through pharmacological or probiotic intervention using Faecalibacterium prausnitzii extracts

Sun, 2019-09-01 00:00
Chronic leg ulcers are a major health burden globally, increasing in incidence with ageing and morbidity from diabetes. A common mechanism highlighted in chronic hard to heal wounds is exaggerated and sustained inflammation which inhibits the closure and therefore represents a therapeutic target. In the present study, we analyzed the effect of supernatant extracted from two strains of Faecalibacterium prausnitzii, a commensal gut microbiome known to have anti-inflammatory activity. C57BL/6 wild type mice were subjected to full thickness excisional wounds and randomized for treatment by extracts from SPA2 or SPAH strains versus vehicle control.

657 Induction of IL-11 expression leads to persistent abnormal fibroblast activity in cutaneous wound healing

Sun, 2019-09-01 00:00
Injury of the skin can provoke excessive accumulation of extracellular matrix (ECM) leading to a hypertrophic scar or keloid development. However, the mechanism responsible for the persistence of the fibrotic response in a wound healing process is yet not well understood. There is growing evidence that abnormal fibroblast activity mediated by IL-11 signalling plays the pivotal role in systemic organ fibrosis. Therefore, we investigated whether the pathologic activity of myofibroblast-like keloid fibroblast (KFs) are potentiated by hypoxia-induced mesenchymal transition mediated by IL-11.

656 Immune cell profiling of wounds from Epidermolysis bullosa patients

Sun, 2019-09-01 00:00
Epidermolysis bullosa or EB, is a group of genetic diseases characterized by excessive skin fragility causing blisters with minimum trauma. In severe EB patients, blisters can develop into nonhealing wounds, which besides being extremely painful, are the starting point to other more severe – and deadly – clinical manifestations, such as systemic infections and cancer. The current state of research points towards EB being a combination of a heritable skin and inflammatory disease. However, the impact of host immune dysregulation has not been explored in the context of wound resolution.

655 Skullcapflavone II Inhibits Degradation of Type I Collagen by Suppressing MMP-1 Transcription in Human Skin Fibroblasts

Sun, 2019-09-01 00:00
Skullcapflavone II is a flavonoid derived from the root of Scutellaria baicalensis, an herbal medicine used for anti-inflammatory and anti-cancer therapies. We analyzed the effect of skullcapflavone II on the expression of matrix metalloproteinase-1 (MMP-1) and integrity of type I collagen in foreskin fibroblasts. Skullcapflavone II did not affect the secretion of type I collagen but reduced the secretion of MMP-1 in a dose- and time-dependent manner. Real-time reverse transcription-PCR and reporter gene assays showed that skullcapflavone II reduced MMP-1 expression at the transcriptional level.

654 Stimulation and Protection of Cutaneous Sensory Neuron System induced by Forest Honey from Ikaria Island

Sun, 2019-09-01 00:00
Sensory innervation involves nerve endings which are located in dermis and epidermis, reaching the uppermost viable layer of skin. Cutaneous innervation is closely linked with major skin functions and contributes to skin homeostasis. Wound-healing, repair process, sense of touch, perception of thermal stimuli as well as intra-epidermal nerve fibre density on face decrease with aging and are associated with changes in neuronal functions and structures. For centuries, honey has been utilized for wound healing purposes but recently, information regarding honey’s mechanisms of action on wound healing and as a therapeutic agent for skin disorders has accumulated at a rapid pace.

653 Topical use of exosomes from human maternal milk accelerates acute cutaneous wound healing

Sun, 2019-09-01 00:00
Cutaneous wound healing is a complex but highly efficient process. Under certain pathologic conditions healing may be impaired, thus lengthening the process and eventually leading to repeated medical intervention and wound chronicity. Exosomes (secreted extracellular vesicles) are present in biological fluids where they exert a key role in intercellular communication at the tissue, organ and organismal levels. Since human breast milk presents abundant maternal extracellular vesicles (MEVs) with immunomodulatory and pro-regenerative properties, in the present study we aimed to evaluate if topical application of MEVs into open wounds would be beneficial for healing.

652 Efficacy and Safety of Autologous Fibroblast Injection for the Treatment of Acne Scars: A 6-month Prospective, Randomized Controlled Study

Sun, 2019-09-01 00:00
Inducing collagen synthesis and remodeling is crucial to achieving optimal clinical improvement in acne scar treatment. Cell therapy with autologous fibroblast can be an alternative treatment option for depressive acne scars. The objective was to investigate the effect of autologous fibroblast treatment of moderate to severely depressed acne scars, a 6-month prospective, a randomized control study was performed at tertiary medical centers. This study was conducted based on a 6-month, prospective, randomized controlled study to compare the effect of autologous fibroblast injection for atrophic acne scar.

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