Journal Of Investigative Dermatology

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Updated: 1 hour 31 min ago

Skin microbiota perturbations are distinct and disease severity-dependent in hidradenitis suppurativa

Tue, 2019-09-17 00:00
Hidradenitis Suppurativa (HS) is a prevalent and debilitating inflammatory skin disease characterized by painful and recurrent nodules and abscesses, malodorous purulent drainage, and disfiguring sinus tract and scar formation involving intertriginous body sites. Microorganisms have been implicated in HS pathogenesis, and broad-spectrum antimicrobial therapy is one of the mainstays of HS management. However, bacteria have been identified in only ∼50% of HS lesions using conventional culture-based methods and no consistent organism has been cultured from HS lesions.(Brook and Frazier, 1999, Gener et al., 2009, Jemec, 2003, Join-Lambert et al., 2011, Leach et al., 1979)

Interleukin-36 promotes systemic Type-I IFN responses in severe forms of psoriasis

Tue, 2019-09-17 00:00
Psoriasis is an immune-mediated skin disorder associated with severe systemic co-morbidities. While IL-36 is a key disease driver, the pathogenic role of this cytokine has mainly been investigated in skin. Thus, its effects on systemic immunity and extra-cutaneous disease manifestations remain poorly understood.To address this issue, we investigated the consequences of excessive IL-36 activity in circulating immune cells. We initially focused our attention on generalised pustular psoriasis (GPP), a clinical variant associated with pervasive up-regulation of IL-36 signalling.

Staphylococcus aureus Lipoteichoic Acid Initiates a TSLP-Basophil-IL4 Axis in the Skin

Tue, 2019-09-17 00:00
Atopic Dermatitis (AD) is an inflammatory skin disease associated with increased levels of type 2 cytokines. In addition, the skin of AD patients is frequently colonized with Staphylococcus aureus and increased levels of this pathogen are associated with increased disease severity and elevated type 2 allergic responses (Leung et al., 2019). Recent studies have also shown that the onset of S. aureus colonization correlates with the onset of type 2 responses (Meylan et al., 2017, Nakatsuji et al., 2016), however, a mechanism to explain the induction of type 2 responses by S.

Pathogenic CD8+ epidermis-resident memory T cells displace dendritic epidermal T cells in allergic dermatitis

Tue, 2019-09-10 00:00
The skin is our interface with the outside world and consequently it is exposed to a wide range of microbes and allergens. Recent studies have indicated that allergen-specific skin-resident memory T (TRM) cells play a role in allergic contact dermatitis (ACD). However, the composition and dynamics of the epidermal T cell subsets during ACD are not known. Here we show that exposure of the skin to the experimental contact allergen 1-fluoro-2,4-dinitrobenzene (DNFB) results in a displacement of the normally occurring dendritic epidermal T cells (DETC) concomitant with an accumulation of epidermal CD8+CD69+CD103+ TRM cells in mice.

Somatic mosaic NLRP3 mutations and inflammasome activation in late-onset chronic urticaria

Mon, 2019-09-09 00:00
Chronic urticaria is a common skin disorder with heterogeneous causes. In the absence of physical triggers, chronic urticarial rash is called idiopathic or spontaneous. The objective of the current study was to identify the molecular and cellular bases of a disease condition displayed by two unrelated patients aged over 60 years who presented for two decades with a chronic urticaria resistant to standard therapy which occurred in the context of systemic inflammation not triggered by cold. In both patients, a targeted sequencing approach using a next generation technology identified somatic mosaic mutations in NLRP3, a gene encoding a key inflammasome component.

Exploratory Assessment Of Oxygen Flow Assisted Cutaneous Administration Of Methotrexate For Superficial Basal Cell Carcinoma, Mycosis Fungoides And Extramammary Paget’s Disease

Mon, 2019-09-09 00:00
The molecular weight of methotrexate (MTX) makes cutaneous penetration difficult. Oxygen flow could enhance the skin permeation of MTX diluted in the LP3 carrier system. This pilot study aims to assess the efficacy, safety and tolerance of oxygen-flow-administered (OFA)-LP3-MTX3% for treating superficial skin cancers. Patients with superficial basal cell carcinoma (sBCC)(n=12), extramammary Paget’s disease (EMPD)(n=5), mycosis fungoides classic type (CMF) (n=10) and folliculotropic (FMF) (n=6) were included in the study and were treated with 4 weekly applications of OFA-LP3-MTX3%.

IL-1β-primed Mesenchymal Stromal Cells improve epidermal substitute engraftment and wound healing via MMPs and TGF-β1

Mon, 2019-09-09 00:00
Since the 1980s, deep and extensive skin wounds and burns are treated with autologous Split-Thickness Skin Grafts, or Cultured Epidermal Autografts (CEAs) when donor sites are limited. However, the clinical use of CEAs often remains unsatisfactory due to poor engraftment rates, altered wound healing and reduced skin functionality.In the past few decades, Mesenchymal Stromal Cells (MSCs) have raised much attention due to their anti-inflammatory, pro-trophic and pro-remodeling capacities. More specifically, gingival MSCs have been shown to possess enhanced wound healing properties compared to other tissue sources.

CD49fhigh Defines A Distinct Skin Mesenchymal Stem Cell Population Capable of Hair Follicle Epithelial Cell Maintenance

Fri, 2019-09-06 00:00
Dermis harbor distinct mesenchymal stem cell populations, which play equally important roles as epidermal stem cells in skin homeostasis and regeneration. However, to reliably identify and directly isolate the in vivo counterpart of these cells is still challenging. Using the epidermal stem cell marker CD49f, we defined CD49fhigh distinct mesenchymal subpopulation in the dermis. In vitro and in vivo differentiation assays and transcriptome analysis demonstrated that CD49fhigh cells possess neural crest-like cell characteristics.

Coagulation Factor XIII-A Subunit Missense Mutation in the Pathobiology of Autosomal Dominant Multiple Dermatofibromas

Wed, 2019-09-04 00:00
Dermatofibromas are common benign skin lesions, the etiology of which is poorly understood. We identified two unrelated pedigrees in which there was autosomal dominant transmission of multiple dermatofibromas. Whole exome sequencing revealed a rare shared heterozygous missense variant in F13A1 gene encoding factor XIII subunit A, a transglutaminase involved in hemostasis, wound healing, tumor growth, and apoptosis. The variant (p.Lys679Met) has an allele frequency of 0.0002 and is predicted to be a damaging mutation.

Itch and mental health in dermatological patients across Europe: a cross sectional study in 13 countries

Tue, 2019-09-03 00:00
Itch is a highly prevalent and multi-dimensional symptom. We aimed to analyze the association between itch and mental health in dermatological patients. This multi-center study is observational cross-sectional conducted in dermatological clinics across 13 European countries. A total of 3530 patients and 1094 healthy controls were included. Patients were examined clinically. Outcome measures were itch (presence, chronicity and intensity), the Hospital Anxiety and Depression Scale, EQ5D-VAS, sociodemographics, suicidal ideation, stress (negative life events and economic difficulties).

672 A cosmetic serum formulated with botanical extracts targeting causes of slackened skin improves skin elasticity, firmness, wrinkle severity and facial contours

Sun, 2019-09-01 00:00
The appearance of slackened, sagging skin tends to become prevalent in the fourth or fifth decade of life regardless of lifestyle or environmental exposure. The constant effect of gravity over time contributes to the emergence of prominent nasolabial folds and sagging skin along the jawline and neck. As such, a treatment product specifically targeted to lift sagging skin is critical to a skin care regimen. Here, we identified multiple botanical extracts that support 3 specific factors associated with slackened skin in vivo: hyaluronic acid, collagenous fibers, and elastic fibers.

523 MITF-mediated extracellular matrix changes control intratumour heterogeneity in melanoma

Sun, 2019-09-01 00:00
Differential tumour cell behaviour caused by environmental conditions, termed dynamic heterogeneity, is a prime cause of drug resistance and understanding its underlying mechanisms is crucial to design effective therapies. We have demonstrated cell-cycling heterogeneity in both melanoma xenograft tumours and melanoma spheroids. This was characterized by the presence within the same tumour/spheroid of clusters of proliferating cells and clusters of G1-arrested cells. The location of the quiescent zones supported oxygen/nutrient deprivation as the cause of cell cycle arrest and the G1-arrested cells reversed to cycling when cultured under normoxia in 2D cultures.

274 Junctional Epidermolysis Bullosa: Bottom Up Control Of The Skin Barrier?

Sun, 2019-09-01 00:00
Junctional epidermolysis bullosa (JEB) caused by loss of function mutations in basement membrane genes including laminin 332 (Lam332) is among the most severe forms of epidermolysis bullosa. Affected individuals suffer from blistering from birth leading to scarring, granulation tissue and susceptibility to infection. Using RNASeq analysis of cells with knockdown of the α3 chain of Lam332 we identified an upregulated cluster of genes involved in cholesterol synthesis, a fundemental processes in skin important for formation of the epidermal barrier.

187 Polyneoplasia in Dermatological Practice

Sun, 2019-09-01 00:00
Polyneoplasia was described in various oncologic pathology. The joint action of different oncogenic factors is being discussed. The objective of our investigation was to estimate the frequency of polyneoplasia occurrence in dermatological practice. The retrospective analysis of the data of 56 patients(average age 64,4±0,5 years) was performed. 32 patients suffered from basal cell carcinoma(BCC), 15 patients suffered from squamous cell carcinoma(SCC), 9 patients suffered from T-cell cutaneous lymphoma(TCL).

186 Prevalence of dermatosis in a paediatric population attended by medical students in Brazil

Sun, 2019-09-01 00:00
In Brazil, the Medical course comprises six years, including lectures, tutorial lessons, and laboratory/clinical/surgical practices. In the last two years, there is internship in Public Health/Family Medicine, Internal Medicine/ Specialties, Surgery, Gynaecology/Obstetrics and Paediatrics. The present study verified the profile of patients presented in an outpatient clinic for Pediatric Dermatology in Valinhos-SP/Brazil, in order to provide data for teaching and for public actions. One dermatologist supervised the medical students.

185 Infrared and high frequency ultrasound imaging of skin lesions in atopic dermatitis and psoriasis vulgaris

Sun, 2019-09-01 00:00
Atopic dermatitis (AD) and psoriasis vulgaris (PV) are chronic inflammatory skin diseases that are recognized on the base of clinical presentation and anamnesis. The aim of the study was to evaluate the combination of use thermography and skin ultrasound examinations in the assessment of patients with atopic dermatitis and psoriasis vulgaris. We examined 16 patients: 6 with AD and 10 with PV. Every patient was examined and medical history was taken. Subsequently photography, thermography and high frequency ultrasound (33 MHz and 75 MHz) of skin lesions were performed.


Sun, 2019-09-01 00:00

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Sun, 2019-09-01 00:00